TERRAPUB Aqua-BioScience Monographs


Vol. 3 (No. 2), pp. 39-72, 2010 doi:10.5047/absm.2010.00302.0039

Reproductive Biology of Salmoniform and Pleuronectiform Fishes with Special Reference to Gonadotropin-Releasing Hormone (GnRH)

Masafumi Amano

School of Marine Biosciences, Kitasato University, Ofunato, Iwate 022-0101, Japan

(Received on March 31, 2010; Accepted on June 24, 2010; Published online on August 31, 2010)

Abstract: A salmonid fish, masu salmon Oncorhynchus masou, has salmon gonadotropin-releasing hormone (sGnRH) and chicken GnRH-II (cGnRH-II), while a pleuronectiform fish, barfin flounder Verasper moseri, has sGnRH, cGnRH-II and seabream GnRH (sbGnRH). In masu salmon, sGnRH-immunoreactive (ir) cell bodies are scattered from the olfactory nerve through the ventral telencephalon (VT) and the preoptic area (POA). cGnRH-II-ir cell bodies are located in the midbrain tegmentum (MT). sGnRH but not cGnRH-II is detected in the pituitary. sGnRH peptide levels and sGnRH mRNA levels in the VT and the POA increased during gonadal maturation. sGnRH neurons are derived from the olfactory epithelium and migrate into the brain. In barfin flounder, sGnRH-ir, cGnRH-II-ir, and sbGnRH-ir cell bodies are located in the olfactory bulbs and the terminal nerve ganglion (TN), the MT, and the POA, respectively, and these neurons do not migrate in the brain. sbGnRH is detected in the pituitary. sbGnRH mRNA levels in the brain increased during gonadal maturation. Although three GnRH systems exist in the barfin flounder, anatomical distinction between the TN- and the POA-GnRH systems is not clear in masu salmon. Thus, it is suggested that sGnRH neurons in masu salmon play different roles according to the location in the brain.

Keywords: GnRH, brain, pituitary, gonad, radioimmunoassay, immunohistochemistry, in situ hybridization, quantitative PCR, masu salmon, sockeye salmon, barfin flounder


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